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Biuret reagent - Biuret test



We prepare two tubes for each unknown so that if one tube contains too much or too little protein to be measured, the other tube should give us a usable reading.







When we assay a protein sample we lose some of it because the colorimetric reagent destroys the protein in the process.

Glass or polystyrene cheap cuvettes may be used.

Scrape it in if necessary.



Analysis The color is stable, but all readings should be taken within 10 min.

This is one of several ways in which one might describe a solution formula.











Choose informative labels for the axes as you learned previously.



After a short delay the absorbance Abs will read 0.


Biuret Test


For each unknown, tube 1 should contain 0.



If the sample remains blue, then there is no protein.

Label near the top of the tube, so that the label does not obstruct the light passing through the color reagent later.


Description: When we mix color reagent with the standards we obtain a range of color intensities to which to compare the unknowns.

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Proteins are an essential component of every structural component of the human body, including all cells and all organelles within our cells.
When two acids are attached through carbonyl and amino groups, they are called peptide bonds.
Principle In addition to standard liquid handling supplies a visible light spectrophotometer is needed, with maximum transmission in the region of 450 nm.

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    +82reps
    Biuret, Vetec (TM) reagent grade, 98% NCGC00091696-01 NCGC00091696-02 NCGC00257088-01 NCGC00259712-01 DS-12122 B0513 FT-0623139 Biuret (Carbamylurea; Imidodicarbonic diamide) C06555 D78197 A895347 Q414902 W-108725 Urea Related Compound A, United States Pharmacopeia (USP) Reference Standard 3 Chemical and Physical Properties 3.1 Computed Properties
    By: d_rickards|||https://www.facebook.com/public/Mary-Rickards|||https://twitter.com/jamesgrickards|||http://drickards.com/
    +297reps
    1. Add 0.5 mL of solubilized sample, which contains up to 2% detergent and between 0.2 and 4 mg protein, to 2.5 mL of the biuret reagent. 2. Mix, allow to stand at room temperature for 30 minutes. 3. Measure the absorbance at 546 nm against a blank containing 0.5 mL of the corresponding sample buffer and 2.5 mL of the biuret reagent. Analysis Note
    By: eMMoG|||https://www.facebook.com/gomme.emmog|||https://twitter.com/emmog|||https://www.youtube.com/channel/UCpvLWpa4lk2s0bl_TyvYTDg
    +183reps
    Biuret reagent is made of Copper sulphate (CuSO4), sodium hydroxide (NaOH) and sodium-potassium tartrate (also known as Rochelle salt). Despite the name, this reagent does not contain Biuret ( (H2N-CO-)2NH). It is a vital component of Biuret protein assay. Biuret Reagent Preparation
    By: caulay|||https://www.facebook.com/macaulay.cartwright.1|||https://twitter.com/Caulay_|||https://www.youtube.com/channel/UCSdgjn6AQ_XhdKEd5oGEgeA
    +185reps
    The biuret method is based on the fact that proteins (and, as a rule, all substances containing two or more peptidic bonds) react with copper to form a colored complex whose absorption ( λmax =454 nm), in the presence of excess copper, is proportional to the amount of protein present. The reagent is obtained by dissolving 1–5 g of copper (II.
    By: Dustlord|||https://www.facebook.com/dustlord.scottster|||https://twitter.com/Dustlord|||https://www.youtube.com/watch?v=1K6Y65n687s
    +374reps
    1. Add 0.5 mL of solubilized sample, which contains up to 2% detergent and between 0.2 and 4 mg protein, to 2.5 mL of the biuret reagent. 2. Mix, allow to stand at room temperature for 30 minutes. 3. Measure the absorbance at 546 nm against a blank containing 0.5 mL of the corresponding sample buffer and 2.5 mL of the biuret reagent.
    By: CrushHazard|||https://www.facebook.com/CrushHazard/|||https://twitter.com/crushhazard|||https://www.reddit.com/user/CrushHazard
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